8R6A

DTX1 WWE domain in complex with ADP bound to WWE1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	68.080, 34.059, 84.578
Unit cell angles	90.00, 89.98, 90.00

Refinement procedure

Resolution	53.030 - 2.400
R-factor	0.2732
Rwork	0.271
R-free	0.30900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.734
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.680	2.450
High resolution limit [Å]	2.400	2.400
Number of reflections	15556	892
<I/σ(I)>	7.25
Completeness [%]	95.3
Redundancy	2.25
CC(1/2)	0.990	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		278	0.1 M Tris pH 8.0, 25 % v/v PEG MME 350