8R5W
Crystal structure of the three anaphylatoxin-like modules in fibulin-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-20 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.680, 34.510, 106.890 |
| Unit cell angles | 90.00, 94.00, 90.00 |
Refinement procedure
| Resolution | 48.800 - 2.200 |
| R-factor | 0.24544 |
| Rwork | 0.243 |
| R-free | 0.29742 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.532 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 2.000 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 54442 | 2700 |
| <I/σ(I)> | 8.4 | 0.7 |
| Completeness [%] | 99.9 | 99.93 |
| Redundancy | 6.8 | 7.1 |
| CC(1/2) | 0.999 | 0.329 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 0.2 M potassium nitrate, 2 % w/v PEG 3350 |
