8R5N
DTX1 WWE domain in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.876, 33.861, 84.037 |
| Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
| Resolution | 24.580 - 1.812 |
| R-factor | 0.1805 |
| Rwork | 0.178 |
| R-free | 0.22680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.950 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.037 | 1.844 |
| High resolution limit [Å] | 1.812 | 1.812 |
| Rmerge | 1.345 | |
| Number of reflections | 34515 | 1682 |
| <I/σ(I)> | 6.3 | 1.5 |
| Completeness [%] | 97.6 | 96.6 |
| Redundancy | 6.4 | 5.5 |
| CC(1/2) | 0.994 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 278 | .1 M Tris pH 8.0, 25 % v/v PEG MME 350 |
