8R58

The RSK 2 N-terminal kinase domain in complex with BMF (1-19)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976300
Spacegroup name	P 21 21 2
Unit cell lengths	82.309, 88.256, 39.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.130 - 2.310
R-factor	0.2376
Rwork	0.235
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	88.256	3.169	2.496
High resolution limit [Å]	2.308	3.058	2.308
Rmerge	0.199	0.932
Rmeas	0.215
Rpim	0.056
Number of reflections	7716	387	387
<I/σ(I)>	9.3	3.648	1.6
Completeness [%]	89.6	89.15	61.8
Redundancy	12.5	13.34	11.7
CC(1/2)	0.995	0.923	0.369

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	25% PEG 550, 0,1M Tris pH 8.5, 10% isopropanol, 1.25 M NaCl as reservoir