8R52

The complex of Glycogen Phosphorylase with epigallocatechin (EGC).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 43 21 2
Unit cell lengths	125.972, 125.972, 115.169
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.080 - 2.100
R-factor	0.15372
Rwork	0.151
R-free	0.20332
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.537
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC (5.8.0267)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	125.970	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	48116	3979
<I/σ(I)>	20.9	8
Completeness [%]	89.0	91.2
Redundancy	8.5	7.4
CC(1/2)	0.998	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		289	10 mM BES buffer pH 6.7