8R4P
Structure of BabA from Helicobacter pylori strain 17875
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-26 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.613, 96.953, 97.408 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.640 - 2.000 |
| R-factor | 0.2124 |
| Rwork | 0.211 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.461 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.640 | 2.071 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.076 | 0.963 |
| Rmeas | 0.085 | 1.089 |
| Rpim | 0.039 | 0.493 |
| Number of reflections | 35796 | 3574 |
| <I/σ(I)> | 13.83 | |
| Completeness [%] | 98.5 | 99.94 |
| Redundancy | 4.5 | 4.5 |
| CC(1/2) | 0.999 | 0.535 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 0.1 M cadmium chloride 0.1 M sodium acetate pH 4.6 30% (v/v) PEG 400 |
