8R4K

Plastidial phosphorylase Pho1 trimer from Solanum tuberosum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	218.058, 134.907, 123.105
Unit cell angles	90.00, 91.30, 90.00

Refinement procedure

Resolution	123.070 - 3.300
R-factor	0.25653
Rwork	0.254
R-free	0.30038
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.268
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	123.070	3.400
High resolution limit [Å]	3.300	3.300
Number of reflections	52489	4606
<I/σ(I)>	9.5
Completeness [%]	97.9
Redundancy	3
CC(1/2)	0.997	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.1 M Tris-HCl, 20 % w/v PEG 4000