8R4H
Crystal structure of the copper efflux oxidase (CueO) from Hafnia alvei deleted of the Met-rich domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | H 3 |
| Unit cell lengths | 169.273, 169.273, 90.387 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.230 - 2.937 |
| R-factor | 0.2002 |
| Rwork | 0.198 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (26-JUL-2023)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.460 | 3.100 |
| High resolution limit [Å] | 2.937 | 2.940 |
| Number of reflections | 20565 | 3008 |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 99.7 | |
| Redundancy | 10.7 | |
| CC(1/2) | 0.996 | 0.642 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 100 mM CHES/ Sodium hydroxide pH 9.5 200 mM Sodium chloride 1260 mM Ammonium sulfate |
