8R48

Structure of plastidial phosphorylase Pho1 from Solanum tuberosum at 2.2 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976256
Spacegroup name	P 31 2 1
Unit cell lengths	127.107, 127.107, 118.693
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	80.840 - 2.240
R-factor	0.17848
Rwork	0.176
R-free	0.22897
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.918
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	118.690	2.310
High resolution limit [Å]	2.240	2.240
Rmerge	0.160
Number of reflections	53632	4611
<I/σ(I)>	10.4
Completeness [%]	100.0
Redundancy	8.2
CC(1/2)	0.996	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M Tris-HCl ph 8.5, 20 % w/v PEG 4000