8R44

PAS-GAF bidomain of Glycine max phytochrome A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.3
Synchrotron site	BESSY
Beamline	14.3
Temperature [K]	40
Detector technology	PIXEL
Collection date	2021-06-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.896
Spacegroup name	P 1 21 1
Unit cell lengths	55.287, 111.812, 68.273
Unit cell angles	90.00, 92.31, 90.00

Refinement procedure

Resolution	43.841 - 1.580
Rwork	0.175
R-free	0.19450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.623
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.841	1.636
High resolution limit [Å]	1.580	1.580
Number of reflections	112709	11216
<I/σ(I)>	12.84
Completeness [%]	99.6
Redundancy	3.7
CC(1/2)	0.998	0.418

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	0.12 M Ethylene glycols (0.3M diethylene glycol; 0.3M triethylene glycol; 0.3M tetraethylene glycol; 0.3M pentaethylene glycol), 0.1 M Tris (base);BICINE pH 8.5, 30% v/v Precipitant Mix 1 (20% v/v PEG 500 MME; 10 % w/v PEG 20000)