8R40
Crystal structure of diabody CR57 in complex with rabies virus protein G domain III
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-24 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.95373 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 57.200, 69.720, 190.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.280 - 2.700 |
R-factor | 0.2191 |
Rwork | 0.217 |
R-free | 0.25860 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.512 |
Data reduction software | xia2 (3.10.1) |
Data scaling software | xia2 (3.10.1) |
Phasing software | PHENIX (1.19rc6-4061) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.480 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.141 | 1.400 |
Rpim | 0.040 | 0.392 |
Number of reflections | 21737 | 1067 |
<I/σ(I)> | 13.6 | 1.8 |
Completeness [%] | 100.0 | |
Redundancy | 13.4 | 13.4 |
CC(1/2) | 0.999 | 0.689 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | Crystallized in condition containing 0.1 M calcium chloride, 0.1 M imidazole pH 6.5, 10 % (v/v) 2-propanol and 30 % (w/v) PEG1500. |