8R3F

C-terminal Rel-homology Domain of NFAT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000000
Spacegroup name	P 1 21 1
Unit cell lengths	48.775, 40.279, 57.419
Unit cell angles	90.00, 97.25, 90.00

Refinement procedure

Resolution	56.960 - 1.550
R-factor	0.1963
Rwork	0.194
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p7h
RMSD bond length	0.013
RMSD bond angle	1.227
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (dev_4839)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.960	1.620
High resolution limit [Å]	1.547	1.547
Number of reflections	24212
<I/σ(I)>	10.33
Completeness [%]	74.4
Redundancy	2
CC(1/2)	0.990	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	containing 25% PEG 3350, 200 mM Sodium Chloride and 100 mM BIS-TRIS buffer at a pH of 6.5