8R38
BIIG2 anti-integrin Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.95372 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.930, 80.882, 87.469 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.380 - 1.380 |
R-factor | 0.19651 |
Rwork | 0.195 |
R-free | 0.21710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.703 |
Data reduction software | DIALS (2.2.11-gad03a188b) |
Data scaling software | Aimless (0.7.9) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.700 | 1.430 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.096 | 2.875 |
Rmeas | 0.104 | 3.266 |
Rpim | 0.039 | 1.507 |
Number of reflections | 169111 | 8404 |
<I/σ(I)> | 7.6 | 0.23 |
Completeness [%] | 99.6 | 96.4 |
Redundancy | 6.6 | 4.5 |
CC(1/2) | 0.998 | 0.104 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | crystallization solution: of 20% w/v PEG 3350, 0.2 M sodium chloride, 2.5% v/v DMSO protein buffer solution: 25 mM Bis-Tris-HCl, approx. 130 mM NaCl at pH 6.5 |