8R37

Klebsiella pneumoniae fosfomycin-resistance protein (FosAKP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9919
Spacegroup name	P 21 21 21
Unit cell lengths	40.620, 47.030, 149.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.850 - 1.480
R-factor	0.1746
Rwork	0.173
R-free	0.20570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.912
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	149.200	1.510
High resolution limit [Å]	1.480	1.480
Rmeas	0.089	1.452
Number of reflections	48410	2174
<I/σ(I)>	11.8	1.2
Completeness [%]	99.4	92.2
Redundancy	9.8
CC(1/2)	0.999	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	PEG3350 18% w/v, potassium sodium tartrate 0.2 M, MnCl2 6 mM