8R2O
Huntingtin-Q17, 1-66, N-MBP fusion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 278.619, 278.619, 278.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.140 - 3.230 |
| R-factor | 0.16763 |
| Rwork | 0.166 |
| R-free | 0.23764 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.873 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.140 | 3.480 |
| High resolution limit [Å] | 3.230 | 3.230 |
| Rmerge | 0.069 | 0.451 |
| Rmeas | 0.083 | 0.546 |
| Number of reflections | 26757 | 5542 |
| <I/σ(I)> | 12.8 | 2.9 |
| Completeness [%] | 93.0 | 97.1 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.25 | 293 | 0.8 M KH2PO4, 0.8 M NaH2PO4, and 0.1 M TRIS/HCl pH 8.25 |
