8QZW

Crystal structure of deoxyhypusine synthase from Trichomonas vaginalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	I 41 2 2
Unit cell lengths	123.145, 123.145, 263.326
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.420 - 1.680
R-factor	0.1485
Rwork	0.148
R-free	0.15530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.420	1.780
High resolution limit [Å]	1.680	1.680
Rmerge	0.101	2.443
Rmeas	0.104	2.532
Number of reflections	114945	18031
<I/σ(I)>	17.99	0.95
Completeness [%]	99.6	97.7
Redundancy	14.67	14.4
CC(1/2)	0.999	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Proplex G3: 0.1M HEPES pH 7.0; 2M (NH4)2SO4