8QZV

Crystal structure of translation factor eIF5A from Trichomonas vaginalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	34.262, 40.795, 117.024
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.520 - 1.350
R-factor	0.2118
Rwork	0.211
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.404
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.800	1.430
High resolution limit [Å]	1.350	1.350
Rmerge	0.069	2.692
Rmeas	0.072	2.815
Number of reflections	37009	68214
<I/σ(I)>	15.25	15.77
Completeness [%]	99.8	98.1
Redundancy	13.6	11.7
CC(1/2)	1.000	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	0.1 M phosphate/citrate pH=4.2 40 % PEG 300 Directly from crystallization screen