8QXX
HCMV DNA polymerase processivity factor UL44 phosphorylated NLS 410-433 bound to mouse importin alpha 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.850, 89.761, 97.595 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.880 - 1.900 |
| R-factor | 0.1764 |
| Rwork | 0.175 |
| R-free | 0.20420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.066 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.390 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.071 | 0.758 |
| Number of reflections | 55424 | 3510 |
| <I/σ(I)> | 9 | 1.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 4.4 | 3.8 |
| CC(1/2) | 0.997 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | Sodium citrate 0.65M, HEPES pH7.0, DTT 0.01M |
