8QWW
Structure of the amyloid-forming peptide LYIQWL from Tc5b, grown from 10% ethanol without TFA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-13 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 4.874, 42.270, 21.442 |
| Unit cell angles | 90.00, 95.62, 90.00 |
Refinement procedure
| Resolution | 19.050 - 1.500 |
| R-factor | 0.1396 |
| Rwork | 0.136 |
| R-free | 0.17370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.158 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.050 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.101 | 0.567 |
| Number of reflections | 1372 | 132 |
| <I/σ(I)> | 7.5 | 1.3 |
| Completeness [%] | 97.9 | 88 |
| Redundancy | 3.28 | |
| CC(1/2) | 0.997 | 0.727 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION, RECRYSTALLIZATION | 310 | The peptide was dissolved in 10 v/v% ethanol at 0.5 mg/ml concentration and incubated at 310K. |
