8QWP
Structure of p53 cancer mutant Y236C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.154, 71.055, 105.258 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.700 - 2.100 |
R-factor | 0.225057927431 |
Rwork | 0.223 |
R-free | 0.26839 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.007 |
RMSD bond angle | 0.850 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.700 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.181 | 0.949 |
Number of reflections | 28446 | 2331 |
<I/σ(I)> | 6.7 | 2 |
Completeness [%] | 97.8 | 99.3 |
Redundancy | 5.4 | 5.5 |
CC(1/2) | 0.994 | 0.764 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | Protein solution: 5.5-6.0 mg/ml in 25 mM HEPES (pH 7.5), 200 mM NaCl, 0.5 mM TCEP. Reservoir buffer: 19% PEG 3350 (w/v), 5% ethylene glycol (v/v), 0.2 M Na/K tartrate. |