8QWL
Structure of p53 cancer mutant Y163C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9999 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 68.600, 84.500, 65.862 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.250 - 1.650 |
R-factor | 0.168516695002 |
Rwork | 0.167 |
R-free | 0.18929 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.832 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.300 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.069 | 0.666 |
Number of reflections | 23257 | 1107 |
<I/σ(I)> | 13.1 | 2 |
Completeness [%] | 99.6 | 98.7 |
Redundancy | 5.5 | 4 |
CC(1/2) | 0.999 | 0.815 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | Protein solution: 5.5-6.0 mg/ml in 25 mM HEPES (pH 7.5), 200 mM NaCl, 0.5 mM TCEP. Reservoir buffer: 24% PEG 3350 (w/v), 10% ethylene glycol (v/v), 0.2 M sodium malonate (pH 7.0). |