8QVT

Crystal structure of Zea mays cytokinin oxidase/dehydrogenase 5 (ZmCKX5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-11
Detector	PSI PILATUS 6M
Wavelength(s)	0.980100
Spacegroup name	P 21 21 21
Unit cell lengths	45.340, 132.400, 185.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.870 - 1.650
R-factor	0.182
Rwork	0.181
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.900	1.730
High resolution limit [Å]	1.650	1.650
Rmerge	0.092	1.303
Rmeas	0.097	1.379
Rpim	0.031	0.443
Number of reflections	135148	19038
<I/σ(I)>	14	1.4
Completeness [%]	99.9	99.6
Redundancy	9.4	9.5
CC(1/2)	0.999	0.586

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M Li2SO4, 0.1 M Tris-HCl pH 8.5, 30 % PEG 3000