8QU3

NF-YB/C Heterodimer in Complex with a 13-mer NF-YA-derived Peptide Stabilized with C8-Hydrocarbon Linker

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-28
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	44.750, 51.390, 72.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.940 - 1.410
R-factor	0.1672
Rwork	0.166
R-free	0.19910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.319
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.940	1.460
High resolution limit [Å]	1.410	1.410
Rmerge	0.061	1.789
Rpim	0.012	0.402
Number of reflections	31035	2923
<I/σ(I)>	26.1	1.4
Completeness [%]	93.4	89.9
Redundancy	24.8	20.3
CC(1/2)	1.000	0.747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293.15	100 mM Morpheus Buffer system 3 pH 9.0, 30% (v/v) Morpheus percipitant mix 1, 100 mM Morpheus carboxylic acids.