8QTY

LytR LCP domain from Streptococcus dysgalactiae subs. dysgalactiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.966
Spacegroup name	I 4 2 2
Unit cell lengths	141.271, 141.271, 133.742
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.608 - 2.800
Rwork	0.271
R-free	0.29270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.968
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MrBUMP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.608	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	16955	2436
<I/σ(I)>	15.5
Completeness [%]	99.9
Redundancy	8
CC(1/2)	0.999	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	2.2 M ammonium sulphate 20% glycerol