8QTO
CRYSTAL STRUCTURE OF HOLO-L28H-FNR OF A. FISCHERI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98400 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 75.200, 75.200, 217.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.770 - 2.400 |
| R-factor | 0.1993 |
| Rwork | 0.198 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.573 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.770 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.071 | 3.408 |
| Number of reflections | 12709 | 1205 |
| <I/σ(I)> | 18.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 11.6 | |
| CC(1/2) | 1.000 | 0.449 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 298 | MPD, HEPES, anaerobic |
