8QSV
Bi-allelic variants of SPOUT1, a novel RNA methyltransferase, cause chromosome missegregation and a rare neurodevelopmental disease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-05 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.070, 80.830, 135.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.010 - 2.620 |
| R-factor | 0.20662 |
| Rwork | 0.203 |
| R-free | 0.28020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.583 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.010 | 2.880 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.086 | 0.663 |
| Rmeas | 0.093 | 0.720 |
| Rpim | 0.036 | 0.276 |
| Number of reflections | 12626 | 3066 |
| <I/σ(I)> | 16.11 | 3.81 |
| Completeness [%] | 99.1 | 98.02 |
| Redundancy | 6.5 | 6.6 |
| CC(1/2) | 0.998 | 0.947 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.06 M MgCl2, CaCl2 or 0.09 M NaF, NaBr, NaI with 0.1 M Imidazole, MES (acid) pH 6.5 and 30 % Ethylene glycol, PEG 8K |
