8QRZ

Microbacterium testaceum C-glucosyl deglycosidase (CGD), wild type

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-16
Detector	DECTRIS EIGER2 XE CdTe 9M
Wavelength(s)	0.61992
Spacegroup name	P 1
Unit cell lengths	78.220, 80.410, 82.960
Unit cell angles	90.17, 94.39, 106.58

Refinement procedure

Resolution	39.910 - 2.000
R-factor	0.2373
Rwork	0.236
R-free	0.28990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.984
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.290	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.111	1.034
Number of reflections	128896	17550
<I/σ(I)>	8.4	1.5
Completeness [%]	98.8	98.7
Redundancy	4.1	3.9
CC(1/2)	0.995	0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M PIPES pH 7.0, 25% (w/v) PEGSH (PEG SMEAR HIGH), 0.1 M rubidium(I) chloride, 0.1 M magnesium(II) formate