8QRY

Crystal structure of the yeast spindle body component Spc98

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-08-17
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5
Spacegroup name	P 21 21 21
Unit cell lengths	28.304, 45.284, 65.569
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.260 - 1.870
R-factor	0.2413
Rwork	0.238
R-free	0.30170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.045
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	MOLREP
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.570	1.900
High resolution limit [Å]	1.870	1.870
Rmeas	0.124	0.434
Number of reflections	7339	282
<I/σ(I)>	8.7
Completeness [%]	99.2
Redundancy	3.3
CC(1/2)	0.981	0.596

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Morpheus B4: PEG 3350/PEG 1K/MPD (1:1:1) 37.5%, MES/imidazole 0.1M pH 6.5, Morpheus Halides 90 mM