Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-11 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.659, 108.128, 124.765 |
| Unit cell angles | 90.00, 95.96, 90.00 |
Refinement procedure
| Resolution | 124.091 - 2.300 |
| Rwork | 0.216 |
| R-free | 0.26290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.605 |
| Data reduction software | autoPROC (1.1.7 (20/04/21)) |
| Data scaling software | Aimless (0.7.9 (10/06/22)) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 124.091 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.256 | 2.014 |
| Rmeas | 0.308 | 2.453 |
| Rpim | 0.169 | 1.382 |
| Number of reflections | 70169 | 4548 |
| <I/σ(I)> | 4.3 | 0.6 |
| Completeness [%] | 98.6 | 99.3 |
| Redundancy | 3 | |
| CC(1/2) | 0.970 | 0.170 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293.15 | Crystallization mix: 0.125 M Magnesium acetate 24% PEG 3350 300 mM galactose (in protein storage buffer) Protein storage buffer: 50 mM Tris pH 7.4 200 mM NaCl 1 mM EDTA 3 mM sodium azide |
