Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.380, 41.360, 72.380 |
| Unit cell angles | 90.00, 104.56, 90.00 |
Refinement procedure
| Resolution | 70.056 - 1.346 |
| Rwork | 0.116 |
| R-free | 0.16410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.941 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.060 | 1.380 |
| High resolution limit [Å] | 1.346 | 1.350 |
| Rmerge | 0.070 | 0.622 |
| Rmeas | 0.077 | 0.711 |
| Number of reflections | 49852 | 2329 |
| <I/σ(I)> | 13.42 | 2.28 |
| Completeness [%] | 92.2 | 58.7 |
| Redundancy | 5.35 | 4.1 |
| CC(1/2) | 0.997 | 0.715 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | 1.5 M sodium citrate, 50 mM Tris pH 8.0 |
