8QPX

Crystal structure of Geodia cydonium Immunoglobulin-like domain of Receptor Tyrosine Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 1 21 1
Unit cell lengths	40.910, 32.730, 92.050
Unit cell angles	90.00, 90.82, 90.00

Refinement procedure

Resolution	40.910 - 2.100
R-factor	0.2232
Rwork	0.222
R-free	0.25030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.494
Data reduction software	XDS
Data scaling software	Aimless (1.12.15)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.910	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.035	0.247
Rpim	0.023	0.157
Number of reflections	47524	3990
<I/σ(I)>	17.4	5
Completeness [%]	98.6	98.7
Redundancy	3.3	3.4
CC(1/2)	0.999	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M Ammonium sulfate, 26% w/v PEG4000