8QPM
Structure of methylene-tetrahydromethanopterin reductase from Methanocaldococcus jannaschii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-10 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 96.660, 96.280, 166.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.330 - 1.800 |
Rwork | 0.180 |
R-free | 0.20160 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.330 | 1.864 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.109 | 0.822 |
Number of reflections | 144127 | 14203 |
<I/σ(I)> | 8.3 | |
Completeness [%] | 99.8 | |
Redundancy | 3.8 | |
CC(1/2) | 0.994 | 0.503 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 281 | 35% (v/v) 2-methyl-2,4-pentanediol, 100 mM sodium acetate pH 4.5 |