8QPL

F420-Dependent Methylene-Tetrahydromethanopterin Reductase with F420 from Methanocaldococcus jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	95.910, 95.910, 166.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.220 - 1.900
R-factor	0.2163
Rwork	0.215
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.325
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.049	1.630
Number of reflections	60825	8287
<I/σ(I)>	15.31
Completeness [%]	98.8
Redundancy	1.902
CC(1/2)	0.999	0.569

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	25% (v/v) polyethylene glycol monomethyl ether 550 100 mM MES pH 6.5 10 mM zinc sulfate 2 mM F420