8QPJ
FAD-independent Methylene-Tetrahydrofolate Reductase Mutant E9Q from Mycobacterium hassiacum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | P 43 |
Unit cell lengths | 45.800, 45.800, 254.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.800 - 1.750 |
Rwork | 0.200 |
R-free | 0.23150 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.800 | 1.813 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.088 | 1.050 |
Number of reflections | 51871 | 5183 |
<I/σ(I)> | 8.17 | |
Completeness [%] | 98.7 | |
Redundancy | 3.3 | |
CC(1/2) | 0.997 | 0.431 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 281 | 25 % PEG MME 5000 10 mg/ml Mfr_E9Q 2 mM NAD+ 2 mM CH3-H4F |