8QP6

Crystal structure of Hepatitis C Virus E1 glycoprotein epitope 314-324 scaffold design 1W4K_08 in complex with neutralizing antibody F(ab) fragment IGH526

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0007
Spacegroup name	P 21 21 21
Unit cell lengths	72.320, 121.760, 234.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.630 - 2.590
R-factor	0.234
Rwork	0.232
R-free	0.27310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.583
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18_3861)
Refinement software	PHENIX (1.18_3861)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.630	2.686
High resolution limit [Å]	2.590	2.593
Number of reflections	64890	6325
<I/σ(I)>	11.18	0.74
Completeness [%]	99.4	97.58
Redundancy	13.6	13.7
CC(1/2)	0.997	0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.2 M Ammonium acetate 0.1 M BIS-Tris 5.5 25 % w/v PEG 3350