8QOH
Crystal structure of the kinetoplastid kinetochore protein KKT14 C-terminal domain from Apiculatamorpha spiralis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 291 |
| Detector technology | PIXEL |
| Collection date | 2021-11-27 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.758, 87.583, 71.879 |
| Unit cell angles | 90.00, 93.61, 90.00 |
Refinement procedure
| Resolution | 43.790 - 2.200 |
| R-factor | 0.22 |
| Rwork | 0.219 |
| R-free | 0.23580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.228 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2-3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.660 | 1.990 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmeas | 0.188 | |
| Number of reflections | 41803 | 2243 |
| <I/σ(I)> | 6.9 | 0.7 |
| Completeness [%] | 93.3 | |
| Redundancy | 6.7 | |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | HEPES, sodium chloride, sodium formate, SOKALAN CP 45, TCEP |
