8QO6

OPR3 variant R283D in complex with NADH4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	49.560, 92.850, 89.770
Unit cell angles	90.00, 97.86, 90.00

Refinement procedure

Resolution	45.710 - 2.370
R-factor	0.2349
Rwork	0.234
R-free	0.27550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.807
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER ((dev_4761))
Refinement software	PHENIX ((dev_4761: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.710	2.470
High resolution limit [Å]	2.370	2.370
Number of reflections	32330	4020
<I/σ(I)>	3.52
Completeness [%]	98.5
Redundancy	6.7
CC(1/2)	0.923	0.197

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM Tris-HCl, 50 mM sodium tartrate, 10% PEG8000