8QNU

Metarhizium robertsii Woronin body major protein (HEX-1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 65 2 2
Unit cell lengths	57.650, 57.650, 198.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.930 - 1.720
R-factor	0.1945
Rwork	0.192
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.839
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.100	1.730
High resolution limit [Å]	1.720	1.720
Number of reflections	21819	1065
<I/σ(I)>	21.92
Completeness [%]	99.8
Redundancy	880.6
CC(1/2)	0.999	0.259

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	IN CELL		300	MrHEX-1 crystallized spontaneously in living T. ni High Five cells after recombinant expression using the baculovirus expression system (DH10EmBacY)