8QNS

Crystal structure of murine AIF bound to N-terminal domain of CHCHD4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-30
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.96546
Spacegroup name	P 21 21 21
Unit cell lengths	110.087, 115.619, 192.727
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	99.146 - 3.206
Rwork	0.233
R-free	0.28440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.204
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.146	3.483
High resolution limit [Å]	3.206	3.206
Rmerge	0.303	1.878
Rmeas	0.315	1.951
Rpim	0.086	0.525
Number of reflections	24919	1247
<I/σ(I)>	7.6	1.6
Completeness [%]	93.6	64.6
Redundancy	13.2	13.6
CC(1/2)	0.996	0.656

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	20% PEG 6k, 100 mM Tris-HCl pH 8.0