8QNQ
Structure of the toxin-antitoxin NatRT complex from Pseudomonas aeruginosa. NatTE29D mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2021-05-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.422, 107.706, 93.528 |
| Unit cell angles | 90.00, 104.06, 90.00 |
Refinement procedure
| Resolution | 53.853 - 2.386 |
| R-factor | 0.2356 |
| Rwork | 0.234 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.704 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.720 | 2.420 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.210 | 1.340 |
| Rmeas | 0.240 | 1.450 |
| Rpim | 0.094 | 0.540 |
| Number of reflections | 39257 | 2102 |
| <I/σ(I)> | 11.8 | 2.3 |
| Completeness [%] | 92.5 | 99 |
| Redundancy | 6.9 | |
| CC(1/2) | 0.970 | 0.665 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | in 0.2 M sodium/potassium phosphate pH 7.5, 0.1 M HEPES 7.5, 22.5 % v/v, PEG Smear Medium, 10 % v/v Glycerol from condition G11 of BCS screen (Molecular dimensions) |
