8QNL

Structure of the toxin-antitoxin NatRT complex from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	93.15
Detector technology	PIXEL
Collection date	2021-05-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	55.180, 106.922, 93.499
Unit cell angles	90.00, 104.21, 90.00

Refinement procedure

Resolution	53.461 - 2.270
R-factor	0.2107
Rwork	0.209
R-free	0.25010
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.529
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.600	2.310
High resolution limit [Å]	2.270	2.270
Rmerge	0.097
Rmeas	0.111
Rpim	0.040
Number of reflections	47690	2387
<I/σ(I)>	13
Completeness [%]	98.9	99.6
Redundancy	6.9	7.1
CC(1/2)	0.996	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.25 M di-Ammonium tartrate and 17.8% PEG 3,350 NeXtal (Molecular Dimensions)