8QMX
OPR3 wildtype in complex with NADPH4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03319 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.640, 89.850, 81.130 |
| Unit cell angles | 90.00, 104.92, 90.00 |
Refinement procedure
| Resolution | 47.340 - 1.400 |
| R-factor | 0.1831 |
| Rwork | 0.182 |
| R-free | 0.20290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.376 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_4761: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.340 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 155161 | 5129 |
| <I/σ(I)> | 15.67 | |
| Completeness [%] | 99.0 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.999 | 0.553 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM MES/Tris, 50 mM ammonium sulfate, 12% PEG8000 |
