8QMI
Crystal structure of RNA G2C4 repeats - native model
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-29 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54056 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.759, 46.637, 51.492 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.251 - 1.500 |
| Rwork | 0.168 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.866 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.251 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.116 | 0.553 |
| Rmeas | 0.124 | 0.276 |
| Rpim | 0.044 | 0.276 |
| Number of reflections | 8334 | 1191 |
| <I/σ(I)> | 10 | 2.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.3 | |
| CC(1/2) | 0.990 | 0.846 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | 0.08 M NaCl, 0.02 M Magnesium chloride hexahydrate, 0.04 M Na cacodylate trihydrate pH 6.0, 35% v/v MPD, 0.012 M Spermine tetrahydrochloride |
