8QMD

Human NK cell receptor NKp80, extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 61
Unit cell lengths	183.544, 183.544, 123.668
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	97.607 - 2.900
Rwork	0.221
R-free	0.25720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.413
Data reduction software	XDS (Jan 10, 2022 BUILT=20220820)
Data scaling software	Aimless (0.7.13)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	158.950	158.950	3.000
High resolution limit [Å]	2.900	11.600	2.900
Rmerge	0.641	0.192
Rmeas	0.714	0.213	12.872
Rpim	0.304	0.091	5.579
Number of reflections	49380	783	4590
<I/σ(I)>	3.1
Completeness [%]	93.9
Redundancy	9.2	8.7	9.2
CC(1/2)	0.962	0.985	0.026

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.8M Succinic Acid 0.1M Tris