8QM5
Potential drug binding sites for translation initiation factor eIF4E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.124, 68.877, 63.729 |
| Unit cell angles | 90.00, 96.57, 90.00 |
Refinement procedure
| Resolution | 38.720 - 1.889 |
| R-factor | 0.1956 |
| Rwork | 0.194 |
| R-free | 0.21970 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.8 (16-JUL-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.720 | 1.900 |
| High resolution limit [Å] | 1.889 | 1.890 |
| Rmeas | 0.050 | 0.680 |
| Number of reflections | 32579 | 641 |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.002M DTT 40% PEG 400 0.1M pH=7.5 HEPES/NaOH |
