8QM0
Crystal structure of the pneumococcal Substrate-binding protein AmiA in complex with Peptide 5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.651, 206.756, 96.795 |
Unit cell angles | 90.00, 90.76, 90.00 |
Refinement procedure
Resolution | 49.173 - 1.760 |
R-factor | 0.1586 |
Rwork | 0.157 |
R-free | 0.19830 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.173 | 1.820 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.096 | |
Number of reflections | 213511 | 10351 |
<I/σ(I)> | 12.7 | |
Completeness [%] | 97.5 | |
Redundancy | 6.9 | |
CC(1/2) | 0.998 | 0.790 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Citric Acid and 25% PEG3350 |