8QLX

Magnaporthe grisea Woronin Body Major protein crystallized in cellulo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 65 2 2
Unit cell lengths	57.660, 57.660, 187.890
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.940 - 1.800
R-factor	0.2142
Rwork	0.212
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.051
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.950	1.820
High resolution limit [Å]	1.800	1.800
Number of reflections	18144	855
<I/σ(I)>	25.86
Completeness [%]	100.0
Redundancy	176.9
CC(1/2)	0.999	0.258

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	IN CELL		300	spontaneous in cellulo crystallization inside living T. ni (High Five cells) after recombinant expression using the DH10EmBacY baculovirus system