8QLW

Purpureocillium lilancinum Woronin Body Major protein crystallized in cellulo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 65 2 2
Unit cell lengths	57.100, 57.100, 200.340
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.450 - 1.970
R-factor	0.2038
Rwork	0.200
R-free	0.23610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.654
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.780	1.990
High resolution limit [Å]	1.970	1.970
Number of reflections	14631	684
<I/σ(I)>	18.27
Completeness [%]	100.0
Redundancy	1211
CC(1/2)	0.999	0.192

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	IN CELL		300	spontaneous crystallization in living High Five cells after recombinant expression using baculoviruses