8QLU

Aspergillus fumigatus Woronin Body Major protein crystallized in cellulo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 65 2 2
Unit cell lengths	58.360, 58.360, 190.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.540 - 2.020
R-factor	0.2181
Rwork	0.217
R-free	0.22920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.449
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.350	2.040
High resolution limit [Å]	2.020	2.020
Number of reflections	13432	633
<I/σ(I)>	12.92
Completeness [%]	99.6
Redundancy	245.8
CC(1/2)	0.996	0.219

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	IN CELL		300	spontaneous crystallization in cellulo in T.ni High Five cells after recombinant expression using the DH10EmBacY baculovirus expression system