8QL1

Crystal structure of the human MDN1-MIDAS/NLE1-UBL complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-26
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9625
Spacegroup name	C 1 2 1
Unit cell lengths	237.160, 69.818, 103.183
Unit cell angles	90.00, 91.79, 90.00

Refinement procedure

Resolution	118.520 - 2.300
R-factor	0.2161
Rwork	0.214
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.558
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	118.522	2.380
High resolution limit [Å]	2.300	2.300
Rpim	0.074
Number of reflections	75310	7442
<I/σ(I)>	8.9
Completeness [%]	99.6
Redundancy	6.6
CC(1/2)	0.981	0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M Ammonium sulfate, 18.2% v/v Polyethylene glycol 300, 0.1 M Sodium Acetate, 3.5 M Ammonium chloride